Australian Researchers Develop A Plan For 'Superinfecting' Mosquitoes To Tackle Zika

Wolbachia is a virus that infects many insects, but it doesn't naturally infect the Aedes mosquitoes that transmit Zika, Dengue and Chikungunya. However, Wolbachia can be introduced into populations of Aedes, where it blocks replication of the nasty diseases they carry, and is passed on from females to their offspring, eventually leading to Aedes populations that are disease-free.

But the diseases themselves can develop resistance to Wolbachia, scuppering control efforts. Now, Australian researchers have developed a strategy to stop resistance developing — 'superinfecting' already Wolbachia-infected mosquitoes with additional strains of the virus. The superinfected insects were healthy, and therefore able to pass the superinfection on to the next generation, providing a new weapon in the fight against Aedes-borne disease, they say.

Cameron Simmons from the University of Melbourne in Parkville, Australia, and Scott O’Neill, from Monash University in Clayton, Australia, are involved in these field trials.

Besides examining the effect on Dengue virus disease in human, they, together with colleagues, are also working on further improvements to the use of Wolbachia in Dengue control. One concern is that the virus will mutate and eventually be able to multiply even in Wolbachia-infected Aedes aegypti hosts.

One way to make this harder — at least in principle — is to increase the number or type of Wolbachia parasites in the host cells. To this end, the researchers explored the possibility of "superinfection", namely the secondary infection of mosquitoes that already carry one strain of Wolbachia with a second one.

For a second infection to rapidly spread into a population already carrying one Wolbachia strain, it must benefit from cytoplasmic incompatibility, and the second strain must not reduce the fitness of the mosquitoes. And in order to reduce the risk for the development of viral resistance, mosquito hosts that are co-infected must have higher levels of Wolbachia parasites in the host cells that the virus uses for replication.

The researchers have reported the generation of superinfected Aedes aegypti mosquitoes that fulfil these criteria.

The first Wolbachia strain is called wMel, and it is the one that is currently spreading through wild Aedes aegypti populations as part of the ongoing field trials.

The second strain, called wAlbB, has similar characteristics in singly infected mosquitoes: it grows to relatively high densities in infected mosquitoes and does not diminish their fitness.

A third strain that has also been developed and tested grows to higher densities—and these are associated with stronger inhibition of virus replication — but the infected mosquitoes are less fit and therefore do not spread the infection as efficiently.

The researchers found that Aedes aegypti mosquitoes infected with both Wolbachia strains showed normal fitness and had higher parasite densities than mosquitoes infected with either one of the Wolbachia strains alone. The two parasite types also seemed to populate the same mosquito tissues.

When the researchers compared Dengue virus inhibition in the superinfected mosquitoes with virus in the two singly-infected ones, they found that the superinfected mosquitoes were at least as efficient, and in some tests more, so than singly-infected ones.

For example, when the researchers allowed the mosquitoes to feed on human volunteers with a Dengue virus infection, they found that the superinfected mosquitoes had fewer dengue viruses in their salivary glands, suggesting that they might be less likely to transmit the infection to their next human victims.

Critical for its ability to spread into the existing Aedes aegypti population, the double-infected strain showed cytoplasmic incompatibility with either of the single-infected ones, suggesting that it could 'sweep' quickly across areas that has seen previous release of Wolbachia-infected mosquitoes and transform the population.

"In summary", the researchers say, "the generation and characterisation of a superinfected line with the desired phenotypic effects to replace single wild infections provides a potential mechanism to overcome the emergence of DENV resistance."

"Both Wolbachia strains are stably maintained in the line with minimal mosquito fitness effects. Importantly, DENV replication is inhibited to a greater extent in the superinfected line compared to both parental lines."

"The observed CI [cytoplasmic incompatibility] phenotype induced by the superinfected line is of particular significance as it would enable the line to be released 'on top of' existing wMel or wAlbB field releases in Dengue endemic areas."


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